Clinostomus funduloides, commonly known as rosyside dace, are a minnow species found in western North Carolina where they inhabit areas of narrow, rocky, headwater streams. Their range extends into Ohio, South Carolina, Tennessee, and a few parts of Pennsylvania. Within western North Carolina rosyside dace can be found in almost every water basin and recently have been found in the Upper French Broad (UFB) river basin. Past work on the CYT-B gene of C. funduloides revealed a clade between the Catawba and French Broad samples dating back 2.4 million years. This lead to a conclusion of bait-bucket release as the point of introduction of C. funduloides into the French Broad river basin. Sequencing of the 1140 bp mitochondrial cytochrome-B gene (CYT-B) gene have now been done on four populations of C. funduloides (from the Broad River basin, Catawba river basin, and the French Broad River basin) to gain a better understanding of the relationships among the different populations of C. funduloides. Morphology of C. funduloides is also being taken into account by measuring body depth and counting scales along the lateral line. C. funduloides’ presence in the UFB is intriguing because of its separation from other populations by the Eastern Continental Divide. By looking at genetics and morphology, we will be able to determine the origins of the UFB population and how unique the UFB population is compared to others.