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Tuesday, April 24 • 8:20am - 8:40am
Development Of A Reconstitution Protocol For ATP Synthase With Various Lipids

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H+ transporting ATP synthase is a remarkable molecular machine that catalyzes the synthesis of ATP by use of two coupled motors, F0 and F1. The use of H+ electrochemical gradient drives rotation of the c ring which couples with the F1 domain to induce conformational changes that catalyze the formation of ATP form ADP and inorganic phosphate. The F0 section of ATP synthase is primarily embedded in the membrane, so it is possible that lipid contacts and composition could play a role in proton translocation and function. Cardiolipin, a phospholipid that comprises a significant portion of the inner mitochondrial membrane, residues have been seen using electron cryo-microscopy. These lipid effects could be essential for normal function. A protocol for reconstitution of ATP synthase will be developed, with emphasis on different techniques for detergent removal and liposome recollection.


Tuesday April 24, 2018 8:20am - 8:40am PDT
123 Zeis Hall