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Spring Symposium on UR and Community Engagement has ended
Tuesday, April 24 • 12:00pm - 1:30pm
Glucuronidation Of 3-Phenoxy Benzoic Acid

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3-Phenoxy Benzoic Acid (3-PBA) is a major metabolite of multiple pyrethroids, the active compound in a majority of household insecticides. Pyrethroids act as a neurotoxin and are lethal to many environmentally important insects, can affect aquatic animals such as fish and neurological symptoms and behavioral changes have been seen in mice with developmental exposure to pyrethroids. In vivo metabolism of pyrethroids has not been thoroughly studied in humans, however, animal studies suggest they are commonly metabolized via phase I oxidation and hydrolysis followed by phase II glucuronidation. In this study, the metabolism and inhibition of 3-PBA will be examined using UDP-glucuronosyltransferase (UGT) supersomes. 3-PBA undergoes glucuronidation by UGT1A9 and is expected to fit the Michaelis-Menten kinetic model, with known inhibitors of UGT1A9 decreasing enzymatic activity. This will be determined through kinetic data acquired via High Pressure Liquid Chromatography - Ultraviolet detection (HPLC-UV) analysis of enzymatic assays, which incubate the supersomes with 3-PBA and inhibitor. Acquiring this data will aid in improving our understanding of pyrethroid metabolism and any activity the potential inhibitors have on 3-PBA glucuronidation. Inhibitory activity could lead to an increased half-life of this metabolite in vivo, which could result in negative effects on human health.

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Tuesday April 24, 2018 12:00pm - 1:30pm
Sherrill Center Concourse